Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Nov 11;11(21):7375–7385. doi: 10.1093/nar/11.21.7375

Isolation and translation of mRNA coding for the variant surface antigens of Paramecium.

J Sommerville
PMCID: PMC326489  PMID: 6196720

Abstract

In the poly(A)+RNA isolated from the ciliate Paramecium primaurelia is found a discrete and abundant mRNA species of high molecular weight (corresponding to about 9,000 nucleotides). This mRNA species has size and abundance characteristics that identify it tentatively as the message coding for the variant cell-surface antigens. After microinjection of the high molecular weight mRNA into amphibian oocytes, polypeptides are synthesized that are immunoprecipitated specifically with antibodies directed against the homologous Paramecium antigen. On collecting the culture medium of oocytes microinjected with Paramecium mRNA, newly-synthesized complete antigen molecules (Mr approximately 300,000) can be recovered by immunoprecipitation.

Full text

PDF
7375

Images in this article

7379Image
on p.7379
7381Image
on p.7381
7382Image
on p.7382
7384Image
on p.7384

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berridge M. V., Lane C. D. Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins. Cell. 1976 Jun;8(2):283–297. doi: 10.1016/0092-8674(76)90012-x. [DOI] [PubMed] [Google Scholar]
  2. Bolivar I. Immunological crossreactivity of Paramecium surface antigens: conformational differences account for cell surface specificity. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1003–1007. doi: 10.1073/pnas.80.4.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Glisin V., Crkvenjakov R., Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 1974 Jun 4;13(12):2633–2637. doi: 10.1021/bi00709a025. [DOI] [PubMed] [Google Scholar]
  6. Hansma H. G. The immobilization antigen of Paramecium aurelia is a single polypeptide chain. J Protozool. 1975 May;22(2):257–259. doi: 10.1111/j.1550-7408.1975.tb05861.x. [DOI] [PubMed] [Google Scholar]
  7. JONES I. G. STUDIES ON THE CHARACTERIZATION AND STRUCTURE OF THE IMMOBILIZATION ANTIGENS OF PARAMECIUM AURELIA. Biochem J. 1965 Jul;96:17–23. doi: 10.1042/bj0960017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kloetzel P. M., Whitfield W., Sommerville J. Analysis of reconstruction of an RNP particle which stores 5S RNA and tRNA in amphibian oocytes. Nucleic Acids Res. 1981 Feb 11;9(3):605–621. doi: 10.1093/nar/9.3.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lane C. D., Colman A., Mohun T., Morser J., Champion J., Kourides I., Craig R., Higgins S., James T. C., Applebaum S. W. The Xenopus oocyte as a surrogate secretory system. The specificity of protein export. Eur J Biochem. 1980 Oct;111(1):225–235. doi: 10.1111/j.1432-1033.1980.tb06097.x. [DOI] [PubMed] [Google Scholar]
  10. Macindoe H., Reisner A. H. Adsorption titration as a specific semi-quantitative assay for soluble and bound Paramecium serotypic antigen. Aust J Biol Sci. 1967 Feb;20(1):141–152. [PubMed] [Google Scholar]
  11. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McTavish C., Sommerville J. Macronuclear DNA organization and transcription in Paramecium primaurelia. Chromosoma. 1980;78(2):147–164. doi: 10.1007/BF00328389. [DOI] [PubMed] [Google Scholar]
  13. Preer J. R., Preer L. B., Rudman B. M. mRNAs for the immobilization antigens of Paramecium. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6776–6778. doi: 10.1073/pnas.78.11.6776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reisner A. H., Rowe J., Macindoe H. M. The largest known monomeric globular proteins. Biochim Biophys Acta. 1969;188(2):196–206. doi: 10.1016/0005-2795(69)90066-x. [DOI] [PubMed] [Google Scholar]
  15. Rungger D., Türler H. DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6073–6077. doi: 10.1073/pnas.75.12.6073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. STEERS E., Jr A comparison of the tryptic peptides obtained from immobilization antigens of Paramecium aurelia. Proc Natl Acad Sci U S A. 1962 May 15;48:867–874. doi: 10.1073/pnas.48.5.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sommerville J. Immobilization antigen synthesis in Paramecium aurelia synthesis in a cell-free amino acid incorporating system. Biochim Biophys Acta. 1970 May 21;209(1):240–249. doi: 10.1016/0005-2787(70)90679-9. [DOI] [PubMed] [Google Scholar]
  18. Sommerville J., McTavish C. The effect of temperature change on gene expression in Paramecium primaurelia. Biochim Biophys Acta. 1982 Aug 30;698(2):158–166. doi: 10.1016/0167-4781(82)90131-2. [DOI] [PubMed] [Google Scholar]
  19. Sommerville J. Serotype transformation in Paramecium aurelia: antigen synthesis after a temperature change. Exp Cell Res. 1969 Oct;57(2):443–446. doi: 10.1016/0014-4827(69)90171-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES