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. 2012 Feb;18(2):241–252. doi: 10.1261/rna.030338.111

FIGURE 3.

FIGURE 3.

The role of the CTFV stimulatory RNA stem 1 region in readthrough. (A) Complementary and compensatory changes to base pairs within stem 1 were prepared, targeting either blocks of three consecutive base pairs (based on CTFV-121 and shown to the left of the main stem) or single base pairs (based on CTFV-106 and shown to the right of the main stem). In CTFV121GC, the central GU pair was changed to GC. This mutation was also present in RS1A and S1AFLIP. (B) Plasmids containing the mutations detailed in A were linearized, transcribed, translated, and analyzed according to the legend of Figure 1.