FIGURE 4.

Investigating the role of the CTFV stimulatory RNA loop region in readthrough. Bases within arm 1 of stem 2 (S2, purple), arm 2 of stem 2 and arm 1 of stem 3 (S2/3, blue), or arm 2 of stem 3 (S3, red) were changed to the complementary Watson–Crick bases (e.g., in S2, CGAGAGU was replaced by GCUCUCA) and readthrough measured as detailed in the legend to Figure 1. A deletion mutation was also tested (L7, green), in which most of the main loop was removed (boundaries of the deletion indicated by a dotted line), leaving the seven nucleotides highlighted in green.