FIGURE 5.

Knockdown of hnRNP H/F and TFG showed modest inhibition on alternative splicing of RBFOX2 endogenous targets. HeLa cells were transfected with the indicated siRNAs. After 72 h, total proteins and RNAs were extracted. HeLa cells with stable knockdown of RBFOX2 using shRNA were analyzed in parallel as the positive control. (A) Western blotting analysis using the indicated antibodies. (B) Radioactive RT–PCR of RBFOX1/2 endogenous targets. The targets are classified into three groups: cassette exons induced by RBFOX1/2, cassette exons repressed by RBFOX1/2, and mutually exclusive exons. A representative diagram is shown for each category. The quantified exon-inclusion level is indicated below each panel.