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Analysis of tRNA maturation. RNAs extracted from strains that were wild-type (lane 1) or contained deletions in rph (lane 2), in rnt (lane 3), or in both rph and rnt (lane 4) were analyzed by Northern blot using probes to specific tRNAs. Below each panel, the genes that encode each tRNA as well as the corresponding dinucleotide sequence following the mature tRNA CCA end are shown. The precursors (P) and mature tRNAs (M) that accumulate for the Class I and Class II tRNAs are indicated.
