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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Cancer Res. 2012 Jan;10(1):143–155. doi: 10.1158/1541-7786.MCR-11-0208

Figure 2.

Figure 2

MSI1 mRNA and protein levels are dependent on HuR levels. (A and B) MSI1 mRNA levels are upregulated by HuR. Seventy-two hours after siRNA-mediated silencing of HuR (50 nM) in U251 (A) and U343 (B) glioblastoma cells, MSI1 mRNA was quantified by RT followed by qPCR analysis; β-actin mRNA was utilized as an endogenous control and the data were analyzed using the 2−ΔΔCt methodology. Data were analyzed with Student’s t-test and is presented as the mean ± standard error of the mean. Experiment was performed in triplicate. (C and D) Msi1 protein is positively dependent on HuR levels. By 72 h after siRNA-mediated silencing of HuR (50 nM) in U251 (C) and U343 (D) glioblastoma cells, Msi1 protein levels were assessed by Western blot analysis. Total RNA was extracted using Trizol reagent and cDNA was synthesized using reverse transcriptase. Western blotting of HuR and Msi1 was performed. α-tubulin served as a loading control.