Skip to main content
. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Cancer Res. 2012 Jan;10(1):143–155. doi: 10.1158/1541-7786.MCR-11-0208

Figure 4.

Figure 4

HuR promotes translation of the MSI1 mRNA. Sucrose gradients were used to fractionate lysates from control U251 cells and cells with HuR knockdown. After fractionation of the sucrose gradient post-ultracentrifugation, RNA from each fraction was extracted with TRIzol and analyzed by real-time PCR. GAPDH mRNA, a transcript that is not regulated by HuR, was included as a negative control. Panels (A) and (B) are the results of the A254 during fractionation. The first three peaks from the left are the 40S, 60S, and 80S subunits, while the peaks to the right are the heavier polysomal fractions. In panels (C) and (D), the percentage distribution of the GAPDH (C) or MSI1 (D) is shown.