Fig. 4.

VGLUT2 ablation disrupts piloneural collar development. (A-K) Histological sections of DRG (A,B) or skin (C-K) from E17.5 (A-G) or P0 (H-K) Wt (E), VGLUT2^cKO (A-D,G,H,I,K), VGLUT2^fl/fl (F) or VGLUT2^+/– (J) mice were stained with Hematoxylin and Eosin (H&E) (E-G), co-labeled with antibodies against Nefh and VGLUT2 (A-D) or nestin and NMDAR (H-J) or Nefh alone (K). Panels A,B, C,D and H,I represent the same field of view. Arrowheads identify the location of selected Nefh⁺ DRG neurons (A,B) and associated afferents (C,D) under both fields of view. Asterisks indicate nestin⁺NMDAR⁺ cells (^**) or nestin⁺NMDAR^- cells (^*). Arrows indicate approaching Nefh⁺ fibers that fail to innervate hair follicles (K). Dashed line demarcates the border between the epidermis and dermis (C,D) or hair follicle ORS cells (H-K). 1° Hf, primary guard hair follicle; Derm, dermis; Epid, epidermis; Hf, hair follicle; Peg, secondary hair peg.