Fig. 5.

Frizzled1/2/7 acts through the canonical Wnt pathway and triggers β-catenin nuclearisation specifically in the macromeres. (A) Phenotypes associated with the use of the KTXXXG mutated form of Fz7 (Fz7mut). Fz7mut produced phenotypes similar to DnFz7. (B) Nuclear distribution of endogenous β-catenin proteins at the 32-cell stage in control and DnFz7 embryos. Fz7 signaling is required for nuclear translocation of β-catenin in macromeres but not in micromeres. (C) Nuclear β-catenin activity 8 hours post-fertilization assessed in control, DnFz7 or LiCl-treated embryos using a TOP-Flash reporter assay. Data are mean±s.e.m. of three experiments.