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. 2011 Nov 15;2:58. doi: 10.3389/fimmu.2011.00058

Figure 1.

Figure 1

Binding of HK to gC1qR mutants using solid-phase microplate assay. Duplicate wells of microtiter plates were first coated with 100 μl (2 μg/ml) of wild type or gC1qR mutants (ΔgC1qR). After the unreacted sites were blocked, concentrations of HK were added as indicated. The bound HK was detected by sequential interaction with 2 μg/ml mAb anti-HK, followed by alkaline phosphatase conjugated goat anti-mouse IgG and pNPP. The absorbance of the color developed at the end of the reaction was read at 405 nm using a VMax Kinetic plate reader. Each data point (A,B) is a mean of three experiments run in duplicates and (B) represents a saturation curve of data in (A) and the location in gC1qR of residues 144–162 (C) and 196–202 (D) is provided as a reference. The significance of data is indicated as: *p ≤ 0.05; **p ≤ 0.01 and ***p ≤ 0.001.