Figure 6.

Immunofluorescence studies. Human Brain microvascular endothelial cells (HBr-MIVECs) were first grown on cover slips and the attached cells reacted first with PBS containing 0.1% BSA and 1% heated human serum to block unreacted sites, followed by Alexa-488 conjugated streptavidin or neutravidin. After incubation, the cells were fixed for 10 min with 3.7% (v/v) formalin, and then examined by 3D imaging using deconvolution microscopy. (A) is non-specific control and shows the image of cells reacted with Alexa-conjugated streptavidin (ACS) only. (B–D) show images of cells that were first treated with either biotinylated WT-gC1q-R, or deletion mutants 144–148 or 174–180 and then stained with ACS.