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. Author manuscript; available in PMC: 2012 Jan 24.
Published in final edited form as: Dev Dyn. 2011 Sep;240(9):2175–2193. doi: 10.1002/dvdy.22711

Fig. 11. Re-establishment of the VCM, DCM and hemogenic nascent umbilical artery in allantoic regenerates.

Fig. 11

(A–C) Allantoises were aspirated at EHF-2-s stages (~7.75–8.25 dpc) and immunostained with PECAM-1 (A), afadin (B), or X-gal in the Ptc1:lacZ reporter embryo (C). Arrowheads (A-C) suggest that the primitive streak (ps) may be composed of dorsal (left) and ventral (right) components. (D–G) Initial/final stages, lower left, allantoic regenerates. Afadin-stained VCM (arrowhead, D), alpha-4-integrin-stained DCM (grey arrowhead, E), Ptc-1 in the VCM (arrowhead, F); PECAM-1 (brown)/Runx-1 (royal blue) (arrowhead, H) in the nascent hemogenic vessel of confluence/umbilical artery. Scale bar (G): 50μm (G); 200μm (A–F).