Table 1. PCR primers used in this analysis.

Gene	Forward Sequence 5′-3′	Reverse Sequence 5′-3′	Product Size (bp)	Annealing temp (°C)
Shh	CCCTTTAGCCTACAAGCAGT	CCACTGGTTCATCACAGAG	232	57
Dhh	ACATCACCACGTCTGACC	GAACACCGGTGCAAAGTCAC	309	58
Ihh	ACGTGCATTGCTCTGTCAAGT	CTGGAAAGCTCTCAGCCGGTT	221	58
Ptch1	CTGCTGCTATCCATCAGCGT	AAGAAGGATAAGAGGACAGG	452	58
Ptch2	TGCCTCTCTGGAGGGCTTCC	CAGTTCCTCCTGCCAGTGCA	207	58
Smo	GTGATGATGAGCCCAAGAGA	AGGGGCAGAGTGGTGAAGC	422	58
Gli1	CAGGGAAGAGAGCAGACTGA	AGCTGATGCAGCTGATCCAG	251	60
Gli2	ACGACTTTCTCCACACCCTGC	TGATGTAAGCTACCAGCGAG	478	60
Gli3	ATCCGCTCATTGCACAGCAG	TGAAGCTCAATGCAGGGCTG	436	58
Hprt	CCTGTCGGATTACATTAAAGCACTG	GTCAAGGGCATATCCAACAACAAC	301	57
Gsk3β	CAG CCT TCA GCT TTT GGT AGC	TCCACCAACTGATCCACACCAC	750	58
Pax6	AGTTCTTCGCAACCTGGCTA	TGAAGCTGCTGCTGATAGGA	519	62.5
Su(Fu)	GCT CCA GGT TAC CGC TAT CG	GCT GCC CAA ACT GTC CTTGC	772	58