Figure 1.

Nab2 is differentially regulated in helped versus helpless CD8⁺ T cells. Spleens from TAP^−/−Ad5E1-MEC–primed mice in a helped or helpless setting were harvested and CD8⁺ T cells isolated. A CD44^hi sort was performed on nonreactivated cells. (A) Purified CD8⁺ T cells were restimulated for 4 hours with the E1B_192-200 peptide and intracellular IFNγ staining was performed. Nineteen percent and 10% IFNγ⁺ cells for helped and helpless CD44^hi CD8⁺ T cells was measured, respectively. Gate represents sorting gate employed to enrich for CD44^hi CD8⁺ T cells. (B) Quantitative RT-PCR analysis of Nab2 mRNA levels in restimulated CD44^hi-sorted CD8⁺ T cells. (C) Helped and helpless CD8⁺ T cells isolated from TAP^−/− Ad5E1-MEC–primed mice were restimulated with the E1B_192-200 peptide or left untreated, and the fold induction of Nab2 transcripts was determined. Each connected dataset represents an independently performed experiment (n = 4; T cells were pooled from 4 mice per group). *P < .05; **P < .02.