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. 2011 Nov 29;119(3):798–804. doi: 10.1182/blood-2011-08-373910

Figure 2.

Figure 2

Nab2 blocks TRAIL induction in restimulated helpless CD8+ T cells. CD8+ T cells were purified from TAP−/−Ad5E1-MEC–primed, helpless mice and were transfected with 2 μg of pcDNA3.1-Nab2 or the empty control vector, together with 0.5 μg of the reporter plasmid pMAX-GFP. (A) Twenty-four hours after transfection, GFP expression was determined as a measurement for transfection efficiency. (B-D) CD8+ T cells were cultured for 4 hours in the presence or absence of the E1B192-200 peptide, and the relative expression of TRAIL (B) and IFNγ (D) mRNA was determined. (C) The fold induction of TRAIL in Nab2- or EV-transfected CD8+ T cells was assessed by comparing mRNA levels before and after E1B192-200 peptide restimulation. Each connected dataset represents an independently performed experiment (n = 4; T cells were pooled from 4 mice per group). *P < .05; ***P < .005.