Figure 3.

Transfection of p53 in p53-null SKOV-3 cells. Ovarian cancer SKOV-3 cells were transfected with the plasmid pC53-SN3 or pCMV-Neo-Bam for 24 hr: stable transfection (a) and transient transfection (b). Transfected cells were treated with cisplatin at IC₅₀ dose (75 μM) for 1 hr. The drug was removed by washing the cells with PBS. Cells were then incubated in fresh drug-free media for the indicated hours (3, 6, 12, 24, and 48) up to harvest. Western blot analysis was performed with indicated antibodies. Nontransfected cells were treated with cisplatin alone as control group.