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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Nat Chem Biol. 2011 Sep 25;7(11):818–826. doi: 10.1038/nchembio.670

Figure 3. PU-H71 identifies the aberrant signalosome in CML cells.

Figure 3

(a) Pathway diagram highlighting the PU bead–identified CML signalosome with focus on networks 1 (Raf-MAPK and PI3K-AKT-mTO R pathway), 2 (NF-κB pathway) and 8 (STAT5 pathway). Key nodal proteins in the identified networks are shown in yellow. A detailed list of identified protein networks and component proteins is shown in Supplementary Figure 6 and Supplementary Data Set 1f. (b) Left, representative western blot of a subset of MS-identified protein complexes. No proteins were detected in the control bead pull-downs, and those data are omitted for simplicity of presentation. Right, representative western blot of K562 cells treated for 24 h with vehicle (−) or PU-H71 (+). (c) Representative western blot of single chemical precipitations conducted in primary CML cell extracts with PU and control beads.