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. 2012 Jan 24;6(1):e1469. doi: 10.1371/journal.pntd.0001469

Figure 6. Virulence is recovered after in vitro or in vivo promastigote-amastigote differentiation process.

Figure 6

BMMø were infected at a 1∶10 (cell/parasite) ratio with non-purified promastigotes differentiated from in vitro axenic amastigotes (A) or ex-vivo intracellular amastigotes (B). As a control, a naturally attenuated strain (Aten) was submitted to the same experimental conditions. Data were acquired by FACScalibur cytometer and analyzed by FlowJo software. Three independent experiments were performed; one representative experiment is shown. The mean and standard deviation are shown. *P<0,05; **P<0,01.