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. 2012 Jan 24;7(1):e30357. doi: 10.1371/journal.pone.0030357

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Poché et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Rx-Cre^+/tg mice were crossed to the ROSA26^+/lacZ Cre reporter line and Cre activity was assessed via X-gal staining. Rx-Cre exhibited broad activity throughout the anterior head in such tissues as the retina, tongue, skin, hair follicles, and teeth (A). Closer inspection of the incisors (B and C) and the molars (D and E) revealed that Cre activity was confined to the ameloblast layer (Am) and completely absent from the odontoblast layer (Od). The Cre activity in the molars was more mosaic than the incisors (arrows in E).