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. 2012 Jan 24;7(1):e30372. doi: 10.1371/journal.pone.0030372

Figure 5. BUD-6 dynamics during vegetative hyphal fusion.

Figure 5

(A) BUD-6 became recruited to the tips of homing fusion hyphae, then concentrated at the attachment point and surrounded the opening fusion pore. Shortly after the pore was fully established BUD-6 fluorescence disappeared from this site. Scale bar, 5 µm. See Movie S8 for time course sequence. (B) Transient BUD-6 fluorescence accumulated at incipient fusion sites in the mature colony (arrowhead) and persistent BUD-6 signal at septal pores (all other fluorescently marked sites). Scale bar, 10 µm. (C) SPA-2-GFP became recruited to vegetative hyphal fusion sites (arrow). As it was never seen at completed fusion connections (arrowheads) it must follow the transient dynamics of BUD-6 in this context. Scale bar, 10 µm.