Figure 7. Loss of BUD-6 resulted in a reduced colony extension rate and hyperbranching.

(A) Hyperbranching and polar extension defects resulted in very slowly and extremely dense developing mycelial colonies of Δbud-6, in comparison to the wild type after 24 hours of incubation. (B) Hyphal morphology of wild type and Δbud-6 at the colony margin. Scale bars, 0.5 mm and 0.25 mm, respectively. (C) Quantification of branching frequency, which on average was more than doubled in the mutant compared to the wild type. (D) Comparison of average colony extension speed between Δbud-6 and wild type.