Figure 9. A Δbni-1 strain generated through vegetative homokaryon selection phenocopied growth defects of Δbud-6.

(A and B) Wild-type like phenotype of the heterokaryotic Δbni-1 strain FGSC11490, including conidia and septa (arrows). Scale bars, 50 µm and 10 µm respectively. (C) The lack of septa in the homokaryotic Δbni-1 strain was confirmed by FM4–64 staining. Scale bar 50 µm. (D) FM4–64 staining also confirmed the absence of an organized apical tip growth apparatus, including the Spk. Scale bar, 10 µm. These defects closely resembled phenotypic key features of Δbud-6 (Figures 7 and 8).