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. 2012 Jan 24;7(1):e30648. doi: 10.1371/journal.pone.0030648

Figure 5. MAP morphotypes survive 70°C and are positive for MAP 16SrRNA and IS900.

Figure 5

(A) MAP K-10 log phase and spores, B. subtilis and C. perfringens were heat treated at 70°C for 30 min and subsequently treated with 2% lysozyme, PK, or kanamycin. Heat treated samples were plated on MB7H9 or blood agar and incubated at 37°C under aerobic or anaerobic conditions. (B) Heat treated cultures were plated on blood agar to determine growth of any contaminates. (C) 16SrRNA sequences of germinated heat treated MAP spores compared to reference sequences from MAP, Bacillus spp., Streptomyces spp. and Clostridium spp.. Ten colonies from each plate were selected for sequences. Sequences shown are a consensus from the ten colonies. (D) IS900 duplex PCR of germinated heat treated MAP spores.