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. 2012 Jan 24;7(1):e30648. doi: 10.1371/journal.pone.0030648

Figure 6. Dormant MAP cultures upregulate spore-related transcripts.

Figure 6

(A) A BLAST comparison and reciprocal BLAST searches were conducted between known sporulation genes from Bacillus spp.† and Streptomyces spp.‡ against MAP. Percent similarity was determined by protein alignment. (B) Quantitative real-time PCR was performed on dormant MAP cultures to determine the presence of carD (MAP0475 and MAP0987) and spo0A (MAP1002c). All three genes are upregulated in comparison to log-phase MAP K-10 culture. All samples were conducted in triplicate. C) Multiple sequence alignment of selected CarD proteins. CarD has recently been shown by Stallings et al. to be necessary component of stringency regulation in mycobacteria. Other studies indicate that the stringency response is also necessary for the initiation of sporulation. A multiple sequence alignment of CarD amino acid sequences from mycobacteria and sporulating bacteria was conducted using CLUSTALW.