Table 1. Primers used in this study.
| Gene and direction | Sequence |
| IS900, L1 ± | CCCGTGACAAGGCCGAAGA |
| IS900, L9 ± | CGGCCCTGGCGTTCCTATG |
| IS900, 900 R ŧ | ACGCTGTCTACCACCCCGCA |
| spoIVA, F | AAATCGGCACACGAAAAGTC |
| spoIVA, R | TGCCAATACCGGGATATCAT |
| Clostridium 16SrDNA, F Ψ | AAAGATGGCATCATCATTCAAC |
| Clostridium 16SrDNA, R Ψ | TACCGTCATTATCTTCCCCAAA |
| Universal 16SrRNA, F | AGAGTTTGATCCTGGCTCAG |
| Universal 16SrRNA, R | GGGTGGATCCTCCTT |
| MAP1002c, F | CGGGTGTGGAACTACGACTT |
| MAP1002c, R | TCTTCTTCCTCAGGTACGAGATGT |
| MAP0475, F | GACAAGGTATTCCAGGTGCTG |
| MAP0475, R | CTCGGCGACCTTGTTGAC |
| MAP0987, F | GCACGACGGCATCGTTAT |
| MAP0987, R | GTCAAGTCCGTCCGTCTCGGTGA |
±
Motiwala, A.S., et al. Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis. J Clin Micobiol, 2003. 41(5):2015–26.
ŧ
Bull, T.J., et al. Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing. Microbiology, 2000. 146:3285.
Ψ
Wang, R.F., et al. A 16S rDNA-based PCR method for rapid and specific detection of Clostridium perferingens in food. Mol Cell Probes,m1994. 8(2): 131–7.