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. 2012 Jan 24;7(1):e30684. doi: 10.1371/journal.pone.0030684

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PC12 cells were transiently transfected with SH325-G7-KDEL or the control αphOx-KDEL and harvested at different time points. The p75NTR surface expression levels were determined with mouse anti-p75NTR mAb (MLR2, 1∶200) followed by goat anti-mouse IgG F(ab′)² fragment APC conjugated (1∶200). The blue histograms represent the p75NTR surface expressions of the PC12 cells expressing SH325-G7-KDEL. The white histograms represent the p75NTR surface expressions of the PC12 cells expressing αphOx-KDEL. The efficiency of knockdown p75NTR surface expression is indicated in percent.