Figure 4. CNIH-2 changes glycosylation of GluAs.

A Western blot analysis of total and surface populations of GluA2_i extracted from HeLa cells by surface biotinylation in the absence (CTRL) or presence of CNIH-2 (CNIH-2) (n = 4). Extensive glycosylation of surface GluA2_i during maturation increases its apparent molecular weight. Note the smaller increase upon co-expression of CNIH-2. B Enzymatic deglycosylation analysis of GluA2_i surface populations in the presence (+) or absence (−) of CNIH-2. Surface GluA2_i remained either untreated (C) or was incubated with either endoglycosidase H (H) or PNGase F (F). Note that upon CNIH-2 co-expression, the GluA2_i surface population remains sensitive to endoglycosidase H (n = 2).