Figure 8. BCCIP knockdown leads to neural progenitor cell proliferation and self-renewal defects.

Neural stem and progenitor cells from E15.5 brains of BCCIP-CON and BCCIP-CKD were isolated and cultured in serum-free media to allow neurosphere formation at two seeding concentrations (0.05×10⁶ cells/ml or 0.25×10⁶ cells/ml) . After 7 days in culture, and the cells were counted and re-plated to assess self-renewal ability by culturing for another 7 days. (A) and (B) shows the morphology of neurospheres originated from E15.5 BCCIP-CON and BCCIP-CKD mice. Scale bar = 200 mm.(C) shows the number of neurospheres and (D) shows the total cell numbers grown from the primary culture, panel (E) shows the number of neurospheres formed from re-suspended primary neurospheres to assess the self-renewal capability. The initial concentrations of cells plated are indicated in the figures. *: P<0.05; **: P<0.01; ***: P<0.001. Asterisks indicate significant differences and n indicates the number of individual neuroprogenitor cell lines analyzed.