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. 2012 Jan 24;7(1):e30861. doi: 10.1371/journal.pone.0030861

Figure 1. Aedes albopictus U4.4 cells are Dcr-2 competent and produce two populations of viral small RNAs.

Figure 1

A. Dicer assay in uninfected U4.4 cells. Lane 3 shows processing of a 113-bp dsRNA substrate into 21-nt siRNAs after incubation in a U4.4 cell extract. Synthetic siRNA (21-nt) and input dsRNA (113-nt) are used as size markers in lanes 1 and 2, respectively. B. RNAi reporter assay. Co-transfection of firefly luciferase specific dsRNA with reporter plasmids encoding firefly and Renilla luciferase into U4.4 cells results in silencing of the firefly luciferase reporter. GFP dsRNA was used as non-specific dsRNA control. Renilla luciferase activity was used as internal control to normalize the firefly luciferase activity. Error bars represent the standard deviations of three individual samples. C. Size distribution of the small RNA reads that match the genome of SINV-GFP with 0 mismatches.