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(lanes 1, 2, 3) Transconjugant Leptolyngbya BL0902 clones, (lane 4) E. coli strain AM4353 harboring pRL692, (lane 5) pRL692 DNA, (lane 6) WT Leptolyngbya BL0902, (lane 7) no template DNA, (lane M) 100-bp ladder size marker. Primer pairs used to amplify the plasmid backbone (left) and the Tn5-692 transposon (right) are shown at the bottom.
