. 2012 Jan 24;7(1):e30901. doi: 10.1371/journal.pone.0030901

Table 8. Plasmids and strains.

Strain or plasmid	Derivation and/or relevant characteristic	Source or reference
Plasmids
pAM2255	Expression vector carrying a trc (IPTG inducible) promoter, Ap^r	H. Iwasaki, [70]
pAM4413	pRL1383a vector carrying the yemGFP open reading frame downstream of trc constitutive promoter, Sp^r Sm^r	This study
pAM4418	Conjugal destination vector pRL1383a carrying an IPTG inducible trc promoter upstream of a Gateway recombination cassette, Sp^r Sm^r Cm^r	This study
pAM4418-yemGFP	Expression plasmid resulting from a Gateway LR recombination reaction of pENTR-SD-yemGFP and pAM4418, Sp^r Sm^r	This study
pDEST_M3	Gateway destination vector optimized for Synechococcus elongatus PCC 7942 and carries lacI ^q and trc promoter upstream of a recombination cassette. Ap^r Km^r Cm^r	This study
pENTR-SD/D-TOPO	Gateway entry vector with Shine-Dalgarno sequence; Km^r	Invitrogen
pENTR-SD-yemGFP	pENTR-SD/D-TOPO carrying the yemGFP open reading frame, Km^r	This study
pEXP_1ax-yemGFP	Expression vector carrying yemGFP downstream of an IPTG inducible promoter, Sp^r Sm^r Cm^r	This study
pJS151	Expression plasmid containing yemGFP, a GFP allele with F64L, S65T, and A206K mutations and that has been codon-optimized for expression in yeast. The first two mutations correspond to mut1GFP [71], and the third mutation interferes with GFP dimerization [72]	J. Hasty
pRK2013	Conjugal plasmid, derivative of RK2, Km^r	J. Meeks, [34], [53]
pRL443	Conjugal plasmid, Km^s derivative of RP4, Ap^r Tc^r	[25]
pRL623	Helper plasmid carrying Mob_ColK and methylase genes M.AvaI, M.Eco47II, M.EcoT22I, Cm^r	[25]
pRL692	Transposon mutagenesis plasmid carrying the mobile element Tn5-692 that contains a pMB1 oriV, Sp^r Sm^r Em^r	(GenBank Accession No. AF424805) [36]
pRL1383a	Mobilizable, broad host range plasmid derived from RSF1010, Sp^r Sm^r	(GenBank Accession No. AF403426) [54]

E. coli strains
DH5α	Cloning host	Gibco BRL
DH10B	Cloning host	Gibco BRL
One Shot ccdB Survival™ (T1R)	Cloning host	Invitrogen
One Shot TOP10	Cloning host	Invitrogen
AM1358	DH10B harboring pRL623, Cm^r	[52]
AM1359	DH10B harboring pRL623 and pRL443, Cm^r Ap^r Tc^r	[73]
AM4338	AM1359 harboring pRL1383a, Cm^r Ap^r Tc^r Sp^r Sm^r	This study
AM4353	AM1359 harboring pRL692, Cm^r Ap^r Tc^r Sp^r Sm^r Em^r	This study
AM4389	DH10B harboring pENTR-SD-yemGFP, Km^r	This study
AM4413	DH10B harboring pAM4413, Sp^r Sm^r	This study
AM4414	AM1359 harboring pAM4413, Cm^r Ap^r Tc^r Sp^r Sm^r	This study
AM4415	DH10B harboring pRL443, Ap^r Tc^r	This study
AM4416	DH10B harboring pRK2013, Km^r	This study
AM4417	AM1358 harboring pAM4413, Cm^r Sp^r Sm^r	This study
AM4503	One Shot ccdB Survival™ harboring pDEST_M3, Km^r Ap^r Cm^r	This study
AM4517	DH5α harboring pAM4418-yemGFP, Sp^r Sm^r	This study

Cyanobacterial strains
BL0902	Wild type	This study
BL0909	Wild type	This study
CS-328	Wild type	D. Bryant
PCC 7120	Wild type	Laboratory collection
PCC 7942 (AMC006)	Wild type	Laboratory collection