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. 2012 Jan 24;7(1):e30831. doi: 10.1371/journal.pone.0030831

Figure 4. Increased expression of TNF-α-induces lipolysis in mature adipocytes upon SBM treatment.

Figure 4

(A) SBM-treated (24 h, at 0, 10 and 50 µg/ml) mature adipocytes (8 days) were stained for intracellular lipids with Oil Red O. Retention of Oil Red O within cells was quantitated. (B) Glycerol release into the medium was measured in adipocyte treated with SBM (24 h, at 0, 10 and 50 µg/ml). (C) mRNA expression of the lipolysis-associated target genes perilipin, HSL, PDE3B, Giα1, TNFα, and PPARγ. (D) mRNA expression and secretion of adiponectin. mRNA expression was calculated relative to untreated mature adipocytes after normalization to 18S rRNA. Adiponectin protein in the media was measured by ELISA after treatment for 24 hrs at 0, 10 and 50 µg/ml SBM. Data are expressed as the mean ± SD. *P<0.01 vs. controls. The results were verified by three repetitions of the experiments, which were each conducted in triplicate. “− SBM”  =  cells treated with vehicle, “+ SBM”  =  cells treated with 50 µg/ml SBM.