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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Cell Physiol. 2012 May;227(5):2163–2174. doi: 10.1002/jcp.22950

Fig. 1.

Fig. 1

In vitro expansion of articular chondrocytes on Plastic, ECM, PtoE, or EtoP from P0 to P6. (A) Representative cell morphology from chondrocytes at P0, P1, P4, and P6. (B) Dil-stained chondrocytes (red) with DAPI as a nuclear counterstain (blue) were expanded on either Plastic (Bi) or ECM [immunostained without (Bii) or with (Biii) collagen I antibody (green)]. (C) Expanded cell number was compared for plating on either ECM or Plastic at each passage. (D) Fold-change of collagen II (Col II) mRNA by the mRNA value from native cartilage was compared in cartilage tissue and chondrocytes expanded on ECM or Plastic. (E) Fold-change of collagen I (Col I) mRNA by the mRNA value from native cartilage was compared in cartilage tissue and chondrocytes expanded on ECM or Plastic. Data are shown as average ± SD for n = 4.