Table I. TaqMan® Customized Porcine Marker Gene Primers and Probes.
| Name | Primer/Probe | Sequence (5′-3′) | Applied Biosystem Catalog Number | Gene ID |
|---|---|---|---|---|
| Chondrogenic Markers | ||||
| Aggrecan | Forward | GCCACTGTTACCGCCACTT | AI2Z4PP | 397255 |
| Reverse | CACTGGCTCTCTGCATCCA | |||
| Probe | CTGACCGGGCGACCTG | |||
| Type I Collagen α1 | Forward | CCGTGCCCTGCCAGATC | AI0H8C9 | 397571 |
| Reverse | CAGTTCTTGATTTCGTCGCAGATC | |||
| Probe | TCGCACAACACATTGC | |||
| Type II Collagen α1 | Forward | TCCTGGCCTCGTGGGT | AIJ9V6I | 397323 |
| Reverse | GGGATCCGGGAGAGCCA | |||
| Probe | CTCCCCTGGGAAACC | |||
| Sox9 | Forward | TGGCAAGGCTGACCTGAAG | AI1Q6JH | 396840 |
| Reverse | GCTCAGCTCGCCGATGT | |||
| Probe | CCCCATCGACTTCCGC | |||
| Hypertrophic Markers | ||||
| Type X Collagen α1 | Forward | GGCCCGGCAGGTCATC | AI382VX | 448809 |
| Reverse | TGGGATGCCTTTTGGTCCTT | |||
| Probe | TCAGACCTGGTTCCCC | |||
| ALP | Forward | CCCTTCACTGCCATCCTGTAC | AI1Q8RQ | 100170147 |
| Reverse | CCATGGAGACGTTCTCTCTCTCA | |||
| Probe | ACGGCCCTGGCTACAA | |||
| MMP13 | Forward | AGTTTGGCCATTCCTTAGGTCTTG | AIWRF2U | 397346 |
| Reverse | GGCTTTTGCCAGTGTAGGTATAGAT | |||
| Probe | ACCACTCCAAGGACCC |