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. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: J Mol Biol. 2011 Nov 22;415(4):649–665. doi: 10.1016/j.jmb.2011.11.027

Fig. 3.

Fig. 3

Structure-based sequence alignment of NTF2 family transport proteins. DALI structural alignment of ceNXF2(NTF2) with human (hs) NXF1, S. cerevisiae (sc) Mex67, C. albicans (ca) Mex76, and Rattus norvegicus (rn) NTF2, as well as ceNXT1 with hsNXt1, scMtr2, caMtr2 and rnNTF2. Residues not observable in structures are grey. Secondary structure of ceNXF2(NTF2) and ceNXT1 are shown, and the NXF family insertion and yeast L4 loop insertion are indicated. ceNXF2/ceNXT1 interface direct polar contacts are in red boxes, and residues mutated for pull-down assays are colored red. Blue crosses “+” indicate ceNXF2 and ceNXT1 residues that are structurally equivalent to rnNTF2. Residues in uppercase font for hsNXF1, scMex67, and caMex67 are structurally equivalent to ceNXF2. Residues in uppercase font for hsNXT1, scMtr2, and caMtr2 are structurally equivalent to ceNXT1. Invariant residues are boxed in orange and conserved residues in each subfamily are boxed in yellow. Sequence identities range from 11–30 %, and Cα RMSD values for superposition range from 1.9–2.6 Å for ceNXF2(NTF2) and from 1.5–2.6 Å for ceNXT1 (Table S2). PDB accession codes: rnNTF2 (1OUN), ScMex67/Mtr2 (1OF5), caMex67/Mtr2 (1Q40), hsNXF1/NXT1 (1JKG) and ceNXF2/NXT1 (3NV0).