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. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: J Mol Biol. 2011 Nov 22;415(4):649–665. doi: 10.1016/j.jmb.2011.11.027

Fig. 4.

Fig. 4

Mapping the specificity determinants of NXF/NXT heterodimerization. (a) ceNXF2/ceNXT1 complex shown, highlighting intimacy of the ceNXT1 (violet) and ceNXF2(NTF2) (ESP) interaction. Orientation of ceNXF2(NTF2) in subsequent panels is unchanged. (b) Cartoon representation of ceNXF2(NTF2) (grey) with direct polar contacts (green) and indirect H2O mediated contacts (cyan) shown. Residues N247, D323, Q379, and R389 (all colored green) are also involved in indirect H2O mediated contacts. Inset highlights a cluster of 1 cation-pi interaction and 2 salt bridges, residues labeled. (c) ESP representation comparison of NTF2 domain dimerization interfaces from ceNXF2, hsNXF1 and caMex67. The NXF plug motif, common to all three structures, denoted by black box. Inset of the ceNXF2 NXF plug, highlighting key plug forming residues (labeled in black) and the identities of the ceNXT1 side-chains that pack into the pockets (labeled in yellow). (d) Simple schematic representation of the intricate NXF plug interaction between ceNXF2 and ceNXT1. (e) Position and identity of residues from homologous NXF plug interactions, in same orientation as ceNXF2, with NXF1/Mex67 residues (black) and NXT1/Mtr2 residues (red) indicated.