Skip to main content
. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: J Mol Biol. 2011 Nov 22;415(4):649–665. doi: 10.1016/j.jmb.2011.11.027

Fig. 7.

Fig. 7

The conserved ceNXF2 FG pocket and a novel dimerization-dependent Nup binding pocket are both necessary for colocalization of ceNXF2/ceNXT1 at the nuclear envelope. (a–d) HeLa cell localization/colocalization studies, cells fixed directly in 4% CH2O, permeabilized, and stained with Hoechst 33342 nuclear stain (blue channel). Cell images shown are representative of multiple cells. (a) ceNXF2(L215R/L332Y)-RFP and ceNXT1-EGFP do not colocalize at the nuclear rim. (b) ceNXF2(V327W/S328R/T330R)-RFP and ceNXT1-EGFP do not colocalize at the nuclear rim. (c) ceNXF2-RFP and ceNXT1(E12W/E85R)-EGFP colocalize at the nuclear rim. (d) ceNXT1(E12W/E85R)-EGFP does not localize at the nuclear rim without ceNXF2 present.