Table 2.

Crucial Interface Contacts and Specificity Determinants of NXF/NXT Heterodimerization

ceNXF2	ceNXT1	Binding		ceNXF1	ceNXT1	Binding		hsNXF1	ceNXT1	Binding
wt	wt	+	:::	wt	wt	+	:::	wt	wt	−
R389A	wt	+
R389D	wt	+
S294A	wt	+
S294D	wt	±
H280A	wt	+
H280D	wt	+
D323R	wt	−
D323A	wt	−
Q379K	wt	−
Q379Y	wt	±
wt	K110Q	+	:::	wt	K110Q	+
wt	R134A	+	:::	wt	R134A	±
wt	R134D	±	:::	wt	R134D	−
D323A	R134A	±
D323R	R134D	+
D323R	R134A	−
D323A	R134D	±
wt	D79Y/Q81Y	−
wt	D79A	+	:::	wt	D79A	+
wt	D79H	+	:::	wt	D79H	±
wt	D137A	+	:::	wt	D137A	+
wt	D137R	+	:::	wt	D137R	+
wt	N98V	+	:::	wt	N98V	+	:::	wt	N98V	−
								E533Q	wt	±
								E533Q	N98V	+

Binding activity measured by pull-down and evaluated by SDS-PAGE

“+” means wild type (wt) binding (≥ 80% of wt binding achieved).

“±” means weak/partial binding (~ 25–75% of wt binding achieved).

“−“ means no appreciable binding (≤ 20% of wt binding achieved).