Fig. 5.
RLR signaling is activated after SCI. Representative immunoblot analysis of spinal cord lysates of sham (Sh) and injured rat cords at 30 min, 6h, 24h and 3d after trauma. Spinal cord lysates were immunoblotted with antibodies against (A) Rig-I, (B) MDA5 and (C) VISA, (D) IFNα, IFNβ and the receptor IFN-a-R1. (E) Densitometric analysis of IFN-a-R1 at different time points after SCI. β-actin was used as internal standard and control for protein loading. Data presented as mean +/− SEM. *p < 0.05 and #p < 0.10. n=5.