Table 1.
Characterization of the AuNCs before and after functionalization with HS-PEG-NH2.a
| HS-PEG- NH2 (Dalton) |
Hydrodynamic diameter (nm) |
Zeta potential (mV) |
Total PEGs per AuNCb |
Total PEGs per AuNCc |
Active PEGs per AuNCd |
Active PEGs per AuNCe |
|---|---|---|---|---|---|---|
| no PEG | 96.1 | −47.9 | — | — | — | — |
| 3,000 | 108.1 | 20.3 | 24,700±1,200 | 33,170±2,750 | 8,860±990 | 7,000±3,200 |
| 5,000 | 130.1 | 11.7 | 12,700±3,340 | 20,000±2,400 | 4,760±870 | 3,800±2,100 |
| 20,000 | 113.9 | −35 | 2,100±740 | 3,200±1,770 | 1,010±620 | 760±400 |
a
The edge length of the AuNCs was measured to be 50 nm by TEM
b
Measured by the fluorescamine-based assay
c
Measured by the ninhydrin-based assay
d
Measured by the FITC-labeling assay
e
Measured by the Cu2+-labeling assay