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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Dev Biol. 2011 Dec 13;362(2):282–294. doi: 10.1016/j.ydbio.2011.12.011

Fig. 2.

Fig. 2

The Downstream Element (DE) contributes to activation of a brk enhancer module in Drosophila. A: Diagram of the 0.8-kb “7/8/9” module from upstream of Dm brk (Yao et al., 2008). This fragment (bounded by red boxes) contains three SEs (“S”; the seventh, eighth, and ninth upstream of the transcription start site) plus the DE (“D”) that accompanies the middle SE (#8; see Fig. 1A,C). Wild-type (brk789wt) and DE-mutant (brk789DEm) versions of the fragment are shown. B-H: Expression in late third-instar imaginal disc tissue of GFP reporters driven by either the wild-type (B-E; four independent transgene insertions) or the DE-mutant (F-H; three independent transgene insertions) version of the 7/8/9 module (see A). All transgenes are present in one copy, inserted into the attP2 docking site (see Materials and methods). Mutation of the DE results in severe reduction of the GFP reporter signal.