Table 1.
Vector:cloned cassette | Cloning site | Length of cassette/cloned vector (bp) | Restriction analysis (Kb) | |
---|---|---|---|---|
pGreen II 0000: | EcoRV | BglII | ||
nos::nptII | HpaI | 1,406/7,217 | 7.2 | 4.7 + 2.5 |
35S::nptII | 1,510/7,321 | 7.3 | 4.8 + 2.5 | |
nos::bar | 1,148/6,961 | 6.9 | 4.5 + 2.4 | |
35S::bar | 1,220/7,031 | 7.0 | 4.5 + 2.5 | |
35S::gus | StuI | 2,507/3,304 | 5.8 | 3.3 + 2.5 |
pCAMBIA: | ScaI | |||
35S::nptII | XhoI | 1,685/11,706 | 6.2 + 4.5 + 0.7 + 0.3 | |
35S::bar | 1,371/11,391 | 6.2 + 4.1 + 0.7 + 0.3 |