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Sequence of mouse Cx45 (NCBI: X63100). Amino acid residues in black and red bold font indicate the sequence of the C-terminus included in the six His-Cx45 CT fusion protein analyzed by mass spectrometry. We obtained 89% coverage of this sequence (red). Seven Ser and one Thr residues (highlighted, red underscored) were phosphorylated by CaMKII in vitro as identified by tandem mass spectrometry. Spectra and tables of fragmentation ions are given in the Supplemental Content.
