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. 2012 Jan 10;2:280. doi: 10.3389/fmicb.2011.00280

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Copyright © 2012 Terahara, Yamamoto, Mitsuki, Shibusawa, Ishige, Mizukoshi, Kobayashi and Tsunetsugu-Yokota.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Flow cytometry analysis of HIV-1-infected cells. Unstimulated CD4⁺ T cells were infected with HIV-1_NL-E, HIV-1_NL-D, HIV-1_NLAD8-E, or HIV-1_NLAD8-D and cultured for 5 days (including the initial 1 day culture with TCR-stimulation). (Left panels) representative pseudo-color plot profiles for the Dead⁻/CD3⁺/CD8⁻-gated cell fractions from three donors. Cells were classified as high (red), dull (blue), and negative (black) based on the HIV-1 reporter intensity. (Right panels) histograms of CD4 expression by the high (red), dull (blue), and negative (black) fractions defined.