Skip to main content
. 2011 Sep 1;2(5):276–281. doi: 10.4161/sgtp.2.5.17740

Figure 1.

Figure 1

Raf inhibitor and growth factor treatment induces KSR1/B-Raf binding. (A) KSR1 competes with C-Raf for inhibitor-induced binding to B-Raf. Cycling KSR-/- MEFs and those expressing WT-KSR1 were treated with 10 µM GDC0879 for 1 h. KSR1 or endogenous C-Raf complexes were isolated and examined for endogenous B-Raf. Lysates were analyzed for pERK and KSR levels. (B) Serum-starved HeLa cells were treated with EGF for 5 min. Endogenous KSR1 complexes were isolated and examined for endogenous B-Raf or C-Raf binding.