Figure 6.

Locations of proliferating cells in female adrenal glands at different stages of development. Immunohistochemical staining for Ki67 nuclear antigen (brown nuclear staining) at (A) E18.5, (B) P1, (C) P3, (D) P10 and (E) 8 weeks. Scale bar = 100 µm (A–E are at the same magnification). Staining in the adrenal cortex is primarily in the outer cortex at all stages but more tightly subcapsular at E18.5-P3, while staining in the medulla at E18.5, P1 and P3 is somewhat patchy, as described in the text. Results for Ki67 immunohistochemistry were similar for males and females at each stage studied, so only results for females are illustrated. c, cortex; m, medulla; zf, zona fasciculata; zg, zona glomerulosa; zr, zona reticularis. The square bracket at the top right of each photograph shows the extent of the major Ki67-positive region in the outer cortex at each stage. Brown nuclear staining appears dark in greyscale prints of the figure.