FIGURE 2.

Titration of the redox potential of the disulfide bond of Dtrx and DtrxH33G. Changes in NMR signal intensities for the NH resonances of Cys³⁴ under oxidizing or reducing conditions were reported in the function of half-cell potential of glutathione. Gray triangles and black circles represent signal intensity in the oxidized and reduced states, respectively. Redox potentials were calculated using the Nernst equation from the ratio of concentrations of reduced and oxidized glutathione. Experimental data were fitted against a sigmoidal decay (logistic) function.