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Substitutions L254A/S256A/I258A/D284A (LSID) and R227A in cCPE inhibit cCPE-mediated decrease in TER. MDCK I cells were cultured on Millicell® cell culture inserts, and TER was monitored by CellZscope. Cells were incubated for 18 h with 3.5 μg/ml GST or GST-cCPE constructs. TER values relative to the values before cCPE application were calculated. Results are means ± S.E. (error bars); n = 5. *, p < 0.05 to GST-cCPEWT.
