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IF detection of HSP70 proteins. Expression of HSP70 proteins was investigated in steroid-responsive MCF7 and steroid-resistant BT-549 breast cancer cells, non-breast HeLa cancer cells, and normal HDF-a. The cells were incubated with anti-HSP70 antibodies and then AlexaFluor 488 antibodies for detection of HSP70 proteins (green). Plasma membrane was stained with CM-Dil (red), and nuclei were stained with DAPI (blue). The merged images are also shown.
