TABLE 1.

Summary of the proteins and peptides identified in our AP and MS experiments from MCF7 steroid-responsive breast cancer cells

In the control AP and MS experiments, where we used agarose beads without TDF-P1 peptide for AP, we identified the same cytokeratins 8 and 18, known to be overexpressed in MCF7 cells.

Identified protein	Identified peptides	m/z (charge state)
GRP78 precursor	ITITNDQNR	537.85 (2+)
	VEIIANDQGNR	614.88 (2+)
Heat shock 70-kDa protein 9 (HSPA9)	LLGQFTLIGIPPAPR	797.09 (2+)
	VINEPTAAALAYGLDK	823.59 (2+)
Heat shock 70-kDa protein 8 isoform 1 (HSP8)	VCNPIITK	472.83 (2+)
	VEIIANDQGNR	614.88 (2+)
	TTPSYVAFTDTER	744.51 (2+)
	ITITNDKGR	509.38 (2+)
Heat shock 70-kDa protein 1 (HSP1)	LSKEEIER	502.35 (2+)
	TTPSYVAFTDTER	744.51 (2+)
	ITITNDKGR	509.38 (2+)
	VEIIANDQGNR	614.88 (2+)
α-Tubulin	VGINYQPPTVVGGDLAK	913.18 (2+)
	TIGGGDDSFNTFFSETGAGK	1004.62 (2+)
Cytokeratin 18	IMADIR	359.29 (2+)
	LASYLDR	419.32 (2+)
	AQYDELAR	483.33 (2+)
	VVSETNDTK	496.84 (2+)
	YETELAMR	506.84 (2+)
	VIDDTNITR	521.35 (2+)
	LEAEIATYR	523.87 (2+)
	VKYETELAMR	533.38 (2+)
	KVIDDTNITR	413.96 (3+) 620.44 (2+)
	QSVENDIHGLR	392.29 (3+)
	AQIFANTVDNAR	423.29 (3+) 634.44 (2+)
	GLQAQIASSGLTVEVDAPK	440.65 (3+) 660.47 (2+)
	IVLQIDNAR	942.70 (2+)
Cytokeratin 8	AQYEDIANR	540.32 (2+)
	LALDIEIATYR	639.46 (2+)
	LESGMQNMSIHTK	738.48 (2+)
	VGSSNFR	383.77 (2+)
	ISSSSFSR	435.80 (2+)
	QLETLGQEK	523.38 (2+)
	LVSESSDVLPK	587.43 (2+)
	ASLEAAIADAEQR	672.97 (2+)
	LEGLTDEINFLR	710.49 (2+)